cd34 pe Search Results


pe cd34 rat mab  (Elabscience Biotechnology)
93
Elabscience Biotechnology pe cd34 rat mab
Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, <t>CD34,</t> and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.
Pe Cd34 Rat Mab, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd34 pe antibody  (Novus Biologicals)
91
Novus Biologicals cd34 pe antibody
Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, <t>CD34,</t> and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.
Cd34 Pe Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd105  (Elabscience Biotechnology)
94
Elabscience Biotechnology cd105
Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, <t>CD34,</t> and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.
Cd105, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd34 cell surface markers  (Novus Biologicals)
90
Novus Biologicals cd34 cell surface markers
Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, <t>CD34,</t> and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.
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anti cd34 antibody  (Cedarlane)
93
Cedarlane anti cd34 antibody
Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, <t>CD34,</t> and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.
Anti Cd34 Antibody, supplied by Cedarlane, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd34 antibody conjugated with pe  (Novus Biologicals)
91
Novus Biologicals anti cd34 antibody conjugated with pe
Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, <t>CD34,</t> and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.
Anti Cd34 Antibody Conjugated With Pe, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti cd34 antibodies  (Novus Biologicals)
93
Novus Biologicals anti cd34 antibodies
Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, <t>CD34,</t> and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.
Anti Cd34 Antibodies, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human cd34  (R&D Systems)
93
R&D Systems human cd34
Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, <t>CD34,</t> and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.
Human Cd34, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd34  (R&D Systems)
94
R&D Systems cd34
FIGURE 2. Limited self-renewal of mesenchymal stem/ stromal (MSCs) from breast cancer patients (BCPs). (A) <t>Colony</t> <t>forming</t> <t>units-fibroblastic</t> (CFU-F) size observed for a BCP. (B) CFU-F size observed for a healthy volunteer (HV). Giemsa staining (40 X). (C) CFU-F assay: number of CFU-F observed in BCPs (n = 14) and HVs (n = 7). Values are expressed as mean ± SE. Statistical analysis: unpaired t- test with Welch’s correction. Asterisks indicate a significant difference (***p = 0.0004). (D) Number of stromal cells per microscope optical field (stromal cell density, SCD) in each CFU-F from BM of BCPs (n = 13) and HVs (n = 6). Statistical analysis: nonparametric Mann–Whitney test. Asterisks indicate a significant difference (***p = 0.0040).
Cd34, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nbp2 34713pe  (Novus Biologicals)
91
Novus Biologicals nbp2 34713pe
FIGURE 2. Limited self-renewal of mesenchymal stem/ stromal (MSCs) from breast cancer patients (BCPs). (A) <t>Colony</t> <t>forming</t> <t>units-fibroblastic</t> (CFU-F) size observed for a BCP. (B) CFU-F size observed for a healthy volunteer (HV). Giemsa staining (40 X). (C) CFU-F assay: number of CFU-F observed in BCPs (n = 14) and HVs (n = 7). Values are expressed as mean ± SE. Statistical analysis: unpaired t- test with Welch’s correction. Asterisks indicate a significant difference (***p = 0.0004). (D) Number of stromal cells per microscope optical field (stromal cell density, SCD) in each CFU-F from BM of BCPs (n = 13) and HVs (n = 6). Statistical analysis: nonparametric Mann–Whitney test. Asterisks indicate a significant difference (***p = 0.0040).
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cd34 percp cy5 5  (Novus Biologicals)
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Novus Biologicals cd34 percp cy5 5
FIGURE 2. Limited self-renewal of mesenchymal stem/ stromal (MSCs) from breast cancer patients (BCPs). (A) <t>Colony</t> <t>forming</t> <t>units-fibroblastic</t> (CFU-F) size observed for a BCP. (B) CFU-F size observed for a healthy volunteer (HV). Giemsa staining (40 X). (C) CFU-F assay: number of CFU-F observed in BCPs (n = 14) and HVs (n = 7). Values are expressed as mean ± SE. Statistical analysis: unpaired t- test with Welch’s correction. Asterisks indicate a significant difference (***p = 0.0004). (D) Number of stromal cells per microscope optical field (stromal cell density, SCD) in each CFU-F from BM of BCPs (n = 13) and HVs (n = 6). Statistical analysis: nonparametric Mann–Whitney test. Asterisks indicate a significant difference (***p = 0.0040).
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pe conjugated anti cd34  (Novus Biologicals)
92
Novus Biologicals pe conjugated anti cd34
FIGURE 2. Limited self-renewal of mesenchymal stem/ stromal (MSCs) from breast cancer patients (BCPs). (A) <t>Colony</t> <t>forming</t> <t>units-fibroblastic</t> (CFU-F) size observed for a BCP. (B) CFU-F size observed for a healthy volunteer (HV). Giemsa staining (40 X). (C) CFU-F assay: number of CFU-F observed in BCPs (n = 14) and HVs (n = 7). Values are expressed as mean ± SE. Statistical analysis: unpaired t- test with Welch’s correction. Asterisks indicate a significant difference (***p = 0.0004). (D) Number of stromal cells per microscope optical field (stromal cell density, SCD) in each CFU-F from BM of BCPs (n = 13) and HVs (n = 6). Statistical analysis: nonparametric Mann–Whitney test. Asterisks indicate a significant difference (***p = 0.0040).
Pe Conjugated Anti Cd34, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, CD34, and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.

Journal: Cells

Article Title: IMG-A1: A Novel Immortalized Granulosa Cell Line for Investigating FSH-Dependent Folliculogenesis and Ovarian Pathophysiology

doi: 10.3390/cells14241940

Figure Lengend Snippet: Basic Characterization of the IMG-A1 Granulosa Cell Line. ( a , b ) Phase-contrast micrographs of the IMG-A1 cell culture. ( c ) Staining for senescence-associated β-galactosidase activity in passage 18 cells. ( d – f ) Immunocytochemical staining for the proliferation marker Ki67, showing a phase-contrast image ( d ), Ki67 immunofluorescence ( e ), and a merged image with DAPI nuclear counterstain ( f ). ( g , h ) Representative metaphase spreads showing a normal diploid karyotype (40 chromosomes) ( g ) and a near-tetraploid karyotype (78 chromosomes) from the IMG-A1 line ( h ). ( i – k ) Ploidy analysis by flow cytometry. Control blood cells ( i ) and primary granulosa cells ( j ) show characteristic diploid (2n) and tetraploid (4n, G2/M phase) peaks. The IMG-A1 culture ( k ) displays a predominantly near-tetraploid and near-octaploid cell population. ( l ) Flow cytometry analysis confirming the homogeneity of the IMG-A1 culture (passages 10 and 40) based on staining for the stromal marker CD29 and the absence of hematopoietic markers CD45, CD34, and the fibroblast marker CD90. Quadrant gates were set based on unstained controls. Scale bars = 100 µm.

Article Snippet: PE CD34 Rat mAb [RAM34] , Elabscience Biotechnology Co., Ltd., Wuhan, China , E-AB-F1284D.

Techniques: Cell Culture, Staining, Activity Assay, Marker, Immunofluorescence, Flow Cytometry, Control

FIGURE 2. Limited self-renewal of mesenchymal stem/ stromal (MSCs) from breast cancer patients (BCPs). (A) Colony forming units-fibroblastic (CFU-F) size observed for a BCP. (B) CFU-F size observed for a healthy volunteer (HV). Giemsa staining (40 X). (C) CFU-F assay: number of CFU-F observed in BCPs (n = 14) and HVs (n = 7). Values are expressed as mean ± SE. Statistical analysis: unpaired t- test with Welch’s correction. Asterisks indicate a significant difference (***p = 0.0004). (D) Number of stromal cells per microscope optical field (stromal cell density, SCD) in each CFU-F from BM of BCPs (n = 13) and HVs (n = 6). Statistical analysis: nonparametric Mann–Whitney test. Asterisks indicate a significant difference (***p = 0.0040).

Journal: Oncology research

Article Title: Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients.

doi: 10.32604/or.2023.028104

Figure Lengend Snippet: FIGURE 2. Limited self-renewal of mesenchymal stem/ stromal (MSCs) from breast cancer patients (BCPs). (A) Colony forming units-fibroblastic (CFU-F) size observed for a BCP. (B) CFU-F size observed for a healthy volunteer (HV). Giemsa staining (40 X). (C) CFU-F assay: number of CFU-F observed in BCPs (n = 14) and HVs (n = 7). Values are expressed as mean ± SE. Statistical analysis: unpaired t- test with Welch’s correction. Asterisks indicate a significant difference (***p = 0.0004). (D) Number of stromal cells per microscope optical field (stromal cell density, SCD) in each CFU-F from BM of BCPs (n = 13) and HVs (n = 6). Statistical analysis: nonparametric Mann–Whitney test. Asterisks indicate a significant difference (***p = 0.0040).

Article Snippet: Phenotypic characterization of MSCs from the third subculture MSCs were suspended in PBS containing 1% bovine serum albumin (BSA, A7030, Sigma, Saint Louis, MO, USA), and labeled with the primary Ab against the following human antigens: CD105 (cat. FAB10971V, R&D Systems Inc., Minneapolis, MN, USA), CD90 (cat. FAB2067G, R&D Systems Inc., Minneapolis, MN, USA), CD73 (cat. FAB5795A, R&D Systems Inc., Minneapolis, MN, USA), CD79a (cat. FAB69201G, R&D Systems Inc., Minneapolis, MN, USA), CD11b (cat. FAB1699V, R&D Systems Inc., Minneapolis, MN, USA), and CD34 (cat. FAB7227P, R&D Systems Inc., Minneapolis, MN, USA).

Techniques: Staining, Microscopy, MANN-WHITNEY

FIGURE 3. Altered morphology of mesenchymal stem/stromal (MSCs) from breast cancer patients (BCPs). (A) and (B) MSCs size and shape observed in colony forming units-fibroblastic (CFU-F) assay of a BCP and a healthy volunteer (HV). Giemsa staining (200). (C) Area of stromal cells in typical regions of each CFU-F culture from BCPs (n = 13) and HVs (n = 6). (D) Longitudinal axis of stromal cells in typical regions of CFU-F cultures from BCPs (n = 13) and HVs (n = 6). (E) Horizontal axis stromal cells in typical regions of CFU-F culture from BCPs (n = 13) and HVs (n = 6). The values are expressed as Mean ± SE. Statistical analysis: nonparametric Mann–Whitney test. Asterisks indicate a significant difference (****p < 0.0001 and ***p < 0.0003).

Journal: Oncology research

Article Title: Senescent mesenchymal stem/stromal cells in pre-metastatic bone marrow of untreated advanced breast cancer patients.

doi: 10.32604/or.2023.028104

Figure Lengend Snippet: FIGURE 3. Altered morphology of mesenchymal stem/stromal (MSCs) from breast cancer patients (BCPs). (A) and (B) MSCs size and shape observed in colony forming units-fibroblastic (CFU-F) assay of a BCP and a healthy volunteer (HV). Giemsa staining (200). (C) Area of stromal cells in typical regions of each CFU-F culture from BCPs (n = 13) and HVs (n = 6). (D) Longitudinal axis of stromal cells in typical regions of CFU-F cultures from BCPs (n = 13) and HVs (n = 6). (E) Horizontal axis stromal cells in typical regions of CFU-F culture from BCPs (n = 13) and HVs (n = 6). The values are expressed as Mean ± SE. Statistical analysis: nonparametric Mann–Whitney test. Asterisks indicate a significant difference (****p < 0.0001 and ***p < 0.0003).

Article Snippet: Phenotypic characterization of MSCs from the third subculture MSCs were suspended in PBS containing 1% bovine serum albumin (BSA, A7030, Sigma, Saint Louis, MO, USA), and labeled with the primary Ab against the following human antigens: CD105 (cat. FAB10971V, R&D Systems Inc., Minneapolis, MN, USA), CD90 (cat. FAB2067G, R&D Systems Inc., Minneapolis, MN, USA), CD73 (cat. FAB5795A, R&D Systems Inc., Minneapolis, MN, USA), CD79a (cat. FAB69201G, R&D Systems Inc., Minneapolis, MN, USA), CD11b (cat. FAB1699V, R&D Systems Inc., Minneapolis, MN, USA), and CD34 (cat. FAB7227P, R&D Systems Inc., Minneapolis, MN, USA).

Techniques: Staining, MANN-WHITNEY